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For direct reinforcement of the physical barrier, a synbiotic comprising Clostridium butyricum and chitosan oligosaccharides upregulates tight junction proteins (occludin, claudin-1, ZO-1) and MUC2 while concurrently suppressing TLR4/NF-κB/MAPK signaling, thereby restoring epithelial integrity (Huang et al., 2023)
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Confocal microscopy Synaptic stains HCS sections and fixed mCNs were immunostained with primary antibodies against synaptic markers VGLUT-1 (Synaptic Systems, Göttingen, Germany, Guinea Pig Polyclonal, Cat# 135 304, RRID:AB_2621384, dilution 1:1000) and PSD-95 (Santa Cruz Biotechnology, Dallas, TX, USA, Mouse Monoclonal, Cat# sc-32291, RRID:AB_628113, dilution: 1:50) and secondary Anti-Guinea Pig Alexa Fluor 647 (Invitrogen/Fisher Scientific, Goat Polyclonal, Cat# A-21450, RRID:AB_141882, dilution: 1:500) and Anti-Mouse Alexa Fluor 568 (Invitrogen/Fisher Scientific, Goat Polyclonal, Cat# A-11004, RRID:AB_2534072, dilution: 1:500) antibodies
